People

Prof. Dr. rer. nat. Katja Bühler
Prof. Dr. rer. nat. Katja Bühler


Department of Solar Materials
Helmholtz Centre for Environmental Research
Building 9.1 (SIAB), Room 202
Permoserstraße 15
04318 Leipzig
Tel.: +49-341-235 46 83
Fax.: +49-341-235 45 12 86

E-Mail:

http://tu-dresden.de/die_tu_dresden/fakultaeten/fakultaet_maschinenwesen/ilb/ma/profs
 
 
 
 
 
 
 

 

Research Interests

Development of alternative biotechnological reactor concepts based on in vitro and in vivo approaches


CV / Scientific Career

Education

  Nov 2013      Venia Legendi for microbiology
Title 'Biotechnology & Catalytic Biofilms'
  1998-2001   PhD (Dr. rer. nat.) at the Technical University Hamburg-Harburg, Institut of Biotechnology II with Prof. Rudolf Müller
Title: 'Characterization of a thermostable alkane oxidizing system'
  1993-1998   Academic studies of Biology at the University Hamburg
Focus on Microbiology und Biochemistry. Degree as Dipl. Biol.

Professional Career

  since Dec 2015      Professor for 'Technology of productive Biofilms' at the Technical University Dresden in a joint professorship with the Helmholtzcenter for Environmental Research UFZ, Leipzig, Germany
  2004-2015   Groupleader ‘Technical Enzymology’ Chair of Chemical Biotechnology (Prof. Andreas Schmid),
Dept. Biochemical and Chemical Engineering, TU Dortmund, Germany
  2001-2004   Postdoctoral Researcher Institut of Biotechnology, Prof. Bernard Witholt, ETH Zurich, Switzerland 
  1999-2001   Visiting Scientist Institut of Biotechnology, Prof. Bernard Witholt, ETH Zurich, Switzerland
  1998-2001   Research Scientist Institut of Biotechnology II, TU Hamburg-Harburg, Germany
Publications
Peer Reviewed Articles
2017
  • Willrodt C.*, Halan B.*, Karthaus L., Rehdorf J. , Julsing M. K., Bühler K. and Schmid A. (2017)
    Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow.
    Biotechnology and Bioengineering, 114(2):281–290, DOI-Link
    * These authors contributed equally to this work.
  • Halan B., Vassilev I., Lang K., Schmid A. and Bühler K. (2017)
    Growth of Pseudomonas taiwanensis VLB120ΔC biofilms in the presence of n-butanol.
    Microbial Biotechnology, 10(4):745–755, DOI-Link
  • Schmutzler K., Kupitz K., Schmid A. and Bühler K. (2017)
    Hyperadherence of Pseudomonas taiwanensis VLB120ΔC increases productivity of (S)-styrene oxide formation.
    Microbial Biotechnology, 10(4):735–744, DOI-Link
2016
  • Karande R., Schmid A. and Bühler K. (2016)
    Applications of multiphasic microreactors for biocatalytic reactions.
    Organic Process Research & Development, 20(2):361-370, DOI-Link
  • Halan B., Karande R., Bühler K. and Schmid A. (2016)
    Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor.
    Journal of Flow Chemistry, 6(1):39–42, DOI-Link
  • Karande R., Debor L., Salamanca D., Bogdahn F., Engesser K.-H., Bühler K. and Schmid A. (2016)
    Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms.
    Biotechnology and Bioengineering, 113(1):52-61, DOI-Link
  • Schmutzler K., Kracht O., Schmid A. and Bühler K. (2016)
    Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120.
    Applied Microbiology and Biotechnology, 100(1):347-360, DOI-Link
2015
  • Schmutzler K., Schmid A. and Bühler K. (2015)
    A three step method for analysing bacterial biofilm formation under continuous medium flow.
    Applied Microbiology and Biotechnology, 99(14):6035-6047, DOI-Link
  • Lang K., Bühler K. and Schmid A. (2015)
    Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms.
    Advanced Synthesis and Catalysis, 357(8):1919-1927, DOI-Link
  • David C., Bühler K. and Schmid A. (2015)
    Stabilization of single species Synechocystis biofilms by cultivation under segmented flow.
    Journal of Industrial Microbiology & Biotechnology, 42(7):1083-1089, DOI-Link
2014
  • Tomaszewski B., Schmid A. and Bühler K. (2014)
    Biocatalytic production of catechols using a high pressure tube-in-tube segmented flow microreactor.
    Organic Process Research & Development, 18(11):1516–1526, DOI-Link
  • Ruinatscha R., Bühler K. and Schmid A. (2014)
    Development of a high performance electrochemical cofactor regeneration modul and its application for the continuos reduction of FAD.
    Journal of Molecular Catalysis B, 103:100–105, DOI-Link
  • Churakova E.*, Tomaszewski B.*, Bühler K., Schmid A., Arends I. W. C. E and Hollmann F. (2014)
    Hydrophobic formic acid esters for cofactor regeneration in aqueous / organic two-liquid phase systems.
    Topics in Catalysis, 57(5):385-391, DOI-Link
    * These authors contributed equally to this work.
  • Lang K., Zierow J., Bühler K. and Schmid A. (2014)
    Metabolic engineering of Pseudomonas sp. VLB120 towards a platform biocatalyst for the production of isobutyric acid and other secondary metabolites.
    Microbial Cell Factories, 13:2, DOI-Link
  • Tomaszewski B., Lloyd R., Warr A., Bühler K. and Schmid A. (2014)
    Regioselective biocatalytic aromatic hydroxylation in a gas-liquid multiphase tube-in-tube reactor.
    ChemCatChem, 6(9):2567–2576, DOI-Link
  • Halan B., Letzel T., Schmid A. and Bühler K. (2014)
    Scalability of a solid support membrane aerated biofilm reactor for the continuous synthesis of (S)-styrene oxide.
    Biotechnology Journal, 9(10):1339-1349, DOI-Link
  • Karande R.*, Halan B.*, Schmid A. and Bühler K. (2014)
    Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.
    Biotechnology and Bioengineering, 111(9):1831-1840, DOI-Link
    * These authors contributed equally to this work.
2013
  • Gross R., Bühler K. and Schmid A. (2013)
    Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide.
    Biotechnology and Bioengineering, 110(2):424–436, DOI-Link
2012
  • Halan B., Bühler K. and Schmid A. (2012)
    Biofilms as living catalysts in chemical synthesis.
    Trends in Biotechnology, 30(9):453-465, DOI-Link
2011
  • Hollmann F., Arends I. W. C. E, Bühler K., Schallmey A. and Bühler B. (2011)
    Enzyme-mediated oxidations for the chemist.
    Green Chemistry, 13:226-265, DOI-Link
  • Ruinatscha R., Karande R., Bühler K. and Schmid A. (2011)
    Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using Sepabead EC-EA and EC-Q1A anion-exchange carriers.
    Molecules, 16(7):5975-5988, DOI-Link
  • Karande R., Schmid A. and Bühler K. (2011)
    Miniaturizing Biocatalysis: Enzyme catalyzed reactions in an aqueous/organic segmented flow capillary microreactor.
    Advanced Synthesis and Catalysis, 353(13):2511-2521, DOI-Link
  • Halan B., Schmid A. and Bühler K. (2011)
    Real time solvent tolerance analysis of Pseudomonas sp. strain VLB120ΔC catalytic biofilms.
    Applied and Enviromental Microbiology, 77(5):1563-1571, DOI-Link
2010
  • Hollmann F., Arends I. W. C. E and Bühler K. (2010)
    Biocatalytic redox reactions for Organic Synthesis: Nonconventional regeneration methods.
    ChemCatChem, 2(7):762-782, DOI-Link
  • Gross R., Lang K., Bühler K. and Schmid A. (2010)
    Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis.
    Biotechnology and Bioengineering, 105(4):705-717, DOI-Link
  • Karande R., Schmid A. and Bühler K. (2010)
    Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
    Langmuir, 26(11):9152-9159, DOI-Link
  • Halan B., Schmid A. and Bühler K. (2010)
    Maximizing the productivity of catalytic biofilms on solid supports in membrane-aerated reactors.
    Biotechnology and Bioengineering, 106(4):516-527, DOI-Link
  • Blank L. M., Ebert B. E., Bühler K. and Bühler B. (2010)
    Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
    Antioxidants & Redox Signaling, 13(3):349-394, DOI-Link, PubMed
2009
  • Rosche B., Li X. Z., Schmid A. and Bühler K. (2009)
    Microbial biofilms: A concept for industrial catalysis?
    TRENDS in Biotechnology, 27(11):636-643, DOI-Link
  • Ruinatscha R., Dusny C., Bühler K. and Schmid A. (2009)
    Productive asymmetric styrene epoxidation based on a next generation electroenzymatic methodology.
    Advanced Synthesis and Catalysis, 351:2505-2515, DOI-Link
2008
  • Tee K. L., Dmytrenko O., Otto K., Schmid A. and Schwaneberg U. (2008)
    A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB).
    Journal of Molecular Catalysis B: Enzymatic, 50(2-4):121-127, DOI-Link
  • Höllrigl V., Hollmann F., Kleeb A., Bühler K. and Schmid A. (2008)
    TADH, the thermostable alcohol dehydrogenase from Thermus sp. ATN1: A versatile new biocatalyst for organic synthesis.
    Applied Microbiology and Biotechnology, 81(2):263-273, DOI-Link, PubMed
2007
  • Höllrigl V., Otto K. and Schmid A. (2007)
    Electroenzymatic asymmetric reduction of rac-3-methylcyclohexanone to (1S,3S)-3-methylcyclohexanol in organic/aqueous media catalyzed by a thermophilic alcohol dehydrogenase.
    Advanced Synthesis & Catalysis, 349(8-9):1337-1340, DOI-Link
  • Gross R., Hauer B., Otto K. and Schmid A. (2007)
    Microbial biofilms: New catalysts for maximising productivty of long-term biotransformations.
    Biotechnology and Bioengineering, 98(6):1123-1134, DOI-Link, PubMed
2006
  • Ruinatscha R.*, Höllrigl V.*, Otto K. and Schmid A. (2006)
    Productivity of selective electroenzymatic reduction and oxidation reactions: Theoretical and practical considerations.
    Advanced Synthesis & Catalysis, 348(15):2015-2026, DOI-Link
    * These authors contributed equally to this work.
2005
  • Hollmann F., Kleeb A., Otto K. and Schmid A. (2005)
    Coupled chemoenzymatic transfer hydrogenation catalysis for enantioselective reduction and oxidation reactions (Corrigendum in Tetrahedron-Asymmetry (2006), 17:867-868).
    Tetrahedron-Asymmetry, 16(21):3512-3519, DOI-Link
2004
  • Otto K., Hofstetter K., Röthlisberger M., Witholt B. and Schmid A. (2004)
    Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component non-heme flavin-diffusible styrene monooxigenase.
    Journal of Bacteriology, 186(16):5292-5302, DOI-Link, PubMed
Books & Bookchapters
2012
  • Bühler K. and Schmid A. (2012)
    Catalytic biofilms: a powerful concept for future bioprocesses.
    in Lear, G. and Lewis, G. Microbial biofilms: Current research and applications.
    Caister Academic Press, Norfolk, UK:193 - 222, ISBN: 978-1-904455-96-7
  • Hollmann F., Bühler K. and Bühler B. (2012)
    Oxidation of Alcohols, Aldehydes, and Acids.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1325-1437, ISBN: 978-3-527-32547-4
  • Bühler B., Bühler K. and Hollmann F. (2012)
    Oxyfunctionalization of C-C-Multiple Bonds.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1269-1324, ISBN: 978-3-527-32547-4
2009
  • Bühler K. and Schmid A. (2009)
    Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
    in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
    Springer, Berlin, Germany, Vol. 1, ISBN: 3540775889
  • Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
    Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
    in Smolke C. D. The metabolic pathway engineering handbook.
    CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0
  • Bühler K. and Schmid A. (2009)
    Synthesis of enantiopure (S)-styrene oxide by selective oxidation of styrene by recombinant E. coli.
    in John Witthall and Peter Sutton Practical Methods in Biocatalysis and Biotransformations.
    John Wiley & Sons Ltd, Chichester, UK:385-390, ISBN: 978-0-470-51927-1, http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0470519274.html
2001
  • Otto K. (2001)
    PhD Thesis: Characterization of a thermostable alkane oxidizing system.
    Technical University Hamburg-Harburg, Hamburg, Germany
Patent applications
2011
  • Schmid A., Karande R. and Bühler K. (2011)
    Segmented Flow Biofilm Reactor.
    WO/2012/152337 PCT/EP2011/057724, filed
Oral Presentations
2013
  • Bühler K., Karande R., Halan B. and Schmid A. (2013)
    Biofilms- a new chapter in Biocatalysis.
    Verfahrenstechnik Kolloquium RWTH Aachen, Aachen, Germany
2012
  • Bühler K., Karande R., Halan B. and Schmid A. (2012)
    Biofilms: a new chapter in biocatalysis.
    VAAM Jahrestreffen, 18. - 21. May 20012, Tübingen, Germany
2009
  • Bühler K., Gross R., Halan B. and Schmid A. (2009)
    Catalytic Biofilms - efficient catalysts for selective oxyfunctionalization of hydrocarbons.
    Biokatalyse: neue Verfahren, neue Produkte, 18. - 20. Mai 2009, Bad Schandau, Germany
  • Bühler K., Gross R., Halan B. and Schmid A. (2009)
    Biofilms as promising catalysts for redoxchemistry.
    Biotrans, Bern, Schweiz
2007
  • Otto K., Gross R., Halan B. and Schmid A. (2007)
    Potential of biofilms for redox biocatalysis.
    Bioperspectives, 30. May - 1. June 2007, Cologne, Germany
Posters
2016
  • Maaß M., David C., Altmann S. and Bühler K. (2016)
    Cyanobacterial biofilms: Exploiting the potential of CO2 as a non-fossil carbon source for the production of high value compounds.
    Biofilms 7, 26. - 28. June 2016, Porto, Portugal
2015
  • Halan B., Karande R., Schmid A. and Bühler K. (2015)
    Scale-up and scale-down of truly continous biofilm based bioprocesses.
    DECHEMA Himmelfahrtstagung, 11. - 13. May 2015, Hamburg-Bergedorf, Germany
  • Bühler K., David C. and Schmid A. (2015)
    Stabilization of single species Synechocystis biofilms by hydrodynamic stress.
    1st International Solar Fuels Conference, 26. April - 01. May 2015, Uppsala, Schweden
  • Halan B., Bühler K. and Schmid A. (2015)
    Biofilms as living catalysts for fine chemical synthesis: process design and scale-up.
    1st Industrial Bioprocess-Development Academy, 15. - 17. April 2015, BASF, Ludwigshafen, Germany
  • Halan B., Karande R., Schmid A. and Bühler K. (2015)
    Industrial biotechnology for sustainable and energy  efficient bioprocesses: miniaturized biofilm based
    continuous fine chemical synthesis    .
    UFZ Energy Days, 24. - 25. February 2015, Leipzig, Germany
2014
  • David C., Grund M., Bühler B., Bühler K. and Schmid A. (2014)
    Applied Biocatalysis & Bio-Artificial Photosynthesis.
    POF III Evaluation Meeting, 4. February 2014, Berlin, Germany
2012
  • Schmutzler K., Schmid A. and Bühler K. (2012)
    Modulation of catalytic Pseudomonas sp. strain VLB120ΔC biofilms.
    Biofilms 5, 10. - 12. December 2012, Paris, France
  • Lang K., Schmid A. and Bühler K. (2012)
    Strain engineering towards an isobutyric acid and isobutanol producing catalytic biofilm.
    ESBES, 23. - 26. September 2012, Istanbul, Turkey
  • Tomaszewski B., Schmid A., Hollmann F. and Bühler K. (2012)
    FDH coupled regeneration in low water media.
    GRC Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Lang K., Schmid A. and Bühler K. (2012)
    Debottlenecking Pseudomonas sp. strain VLB120 for isobutanol synthesis.
    3rd annual CLIB-graduate cluster retreat, 22. - 24. February 2012, Bergisch-Gladbach, Germany
  • Schmutzler K., Volmer J., Halan B., Bühler B., Bühler K. and Schmid A. (2012)
    Solvent tolerance in Pseudomonas sp. strain VLB120 - biofilms vs planktonic cells.
    VAAM Jahrestreffen, 18. - 21. March 2012, Tübingen, Germany
2011
  • Karande R., Bühler K. and Schmid A. (2011)
    Applications of segmented flow microreactors for biocatalysis.
    Biotrends, 8. - 9. December 2011, Dortmund, Germany
  • Tomaszewski B., Lutz J., Bühler K. and Schmid A. (2011)
    From an enzyme to a production process - stepwise design of a microreactor system.
    Marie Curie Researchers Symposium, 25. - 27. September 2011, Warsaw, Poland
  • Tomaszewski B., Lutz J., Bühler K. and Schmid A. (2011)
    Stabilization of biocatalysts for application in segmented flow microcapillary reactors.
    Bioverfahrenstechnik an Grenzflächen , 30. May - 1. June 2011, Potsdam, Germany
  • Bühler K., Halan B. and Schmid A. (2011)
    Biofilm catalysis: Impact of styrene on Pseudomonas sp. strain VLB120 biofilms.
    Bioverfahrenstechnik an Grenzflächen, 30. May - 1. June 2011, Potsdam, Germany
  • Lang K., Bühler K. and Schmid A. (2011)
    Development of an isobutanol producing biofilm catalyst.
    DECHEMA: Bioverfahrenstechnik an Grenzflächen, 30. May 2011, Potsdam, Germany
  • Lang K., Bühler K. and Schmid A. (2011)
    Development of an isobutanol producing biofilm catalyst.
    2nd Annual CLIB-graduate cluster retreat, 7. April 2011, Bonn, Germany
  • Fu J., Falcioni F., Bühler B., Bühler K. and Schmid A. (2011)
    Biochemical characterization of P4Hs: Towards hydroxyproline production by recombinant E. coli..
    DECHEMA Summerschool , September 2011, Bad Herrenalb, Germany
2010
  • Halan B., Letzel T., Bufe R., Bühler K. and Schmid A. (2010)
    Catalytic Biofilms: Real time solvent tolerance analysis and new frontiers in reactor design.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
  • Lang K., Bühler K. and Schmid A. (2010)
    Utilization of biofilms as biocatalysts for chemical synthesis.
    28. DECHEMA Jahrestagung der Biotechnologen und ProcessNet, 23. September 2010, Düsseldorf, germany
  • Karande R., Bühler K. and Schmid A. (2010)
    Asymmetric enzymatic hydrogenation in an aqueous/organic flow capillary microreaktor.
    Bioprozessorientiertes Anlagendesign, 10. - 12. Mai 2010, Nürnberg, Germany
  • Lang K., Bühler K. and Schmid A. (2010)
    Utilization of biofilms as biocatalysts for chemical synthesis.
    1st CLIB international conference: Value generating transformation of renewable carbon sources, 3. March 2010, Düsseldorf, Germany
  • Schmid A., Halan B. and Bühler K. (2010)
    Maximising the productivity of catalytic biofilms on solid supports in membrane bioreactors.
    DECHEMA Bioprozessorientiertes Anlagendesign, 10. - 12. May 2010, Nürnberg, Germany
  • Halan B., Letzel T., Bufe R., Bühler K. and Schmid A. (2010)
    Catalytic biofilms: Real time solvent tolerance analysis and new frontiers in reactor design.
    Gordon Research Conference on Biocatalysis, 11. - 16. July 2010, Smithfield, Rhode Island, USA
2009
  • Halan B., Bühler K. and Schmid A. (2009)
    Catalytic biofilms: novel concept for fine chemical synthesis.
    VAAM, 8. - 11. March 2009, Bochum, Germany
  • Gross R., Lang K., Hermes S., Bühler K. and Schmid A. (2009)
    Characterization and optimization of a novel biofilm reactor for fine chemical synthesis.
    VAAM, 8. - 11. March 2009, Bochum, Germany
  • Karande R., Bühler K. and Schmid A. (2009)
    Multiphase slug flow microreactor for enzyme based redox biocatalysis.
    Dechema, Jahrestagung der Biotechnologen, 8. - 10. September 2009, Mannheim, Germany
  • Gross R., Bühler K. and Schmid A. (2009)
    Selective hydroxylation of alkanes with a membrane biofilm reactor.
    Biotrans, 2009, Bern, Switzerland
2008
  • Bühler K., Gross R., Halan B., Hermes S. and Schmid A. (2008)
    Biofilms: Versatile Biocatalysts for Redox-Catalysis?
    Gordon Research Conference on Biocatalysis, 6. - 11. July 2008, Bryant University, Smithfield, RI, USA
  • Karande R., Bühler K. and Schmid A. (2008)
    Asymmetric Enzymatic Hydrogenation in Capillary Microreactors.
    MSB, 9. - 13. March 2008, Berlin, Germany
2007
  • Ruinatscha R., Otto K. and Schmid A. (2007)
    Integrated concepts for efficient coupling of styrene monooxygenase (StyA) with electrochemical cofactor regeneration.
    Biotrans 2007 - 8th International Symposium on Biocatalysis and Biotransformations, 8. - 13. July 2007, Ovideo, Spain
  • Höllrigl V., Otto K. and Schmid A. (2007)
    Production and characterization of the thermophilic alcohol dehydrogenase from Thermus sp. for biotechnological applications.
    Biotrans 2007 - 8th International Symposium on Biocatalysis and Biotransformations, 8. - 13. July 2007, Ovideo, Spain
  • Höllrigl V., Otto K. and Schmid A. (2007)
    Application of electroenzymatic reductions to organic synthesis.
    European BioPerspectives, 30. May - 1. June 2007, Cologne, Germany
  • Otto K., Gross R., Hauer B. and Schmid A. (2007)
    The potential of biofilms for redox biocatalysis.
    4th ASM Conference on Biofilms, 25. - 29. March 2007, Québec, Canada
2005
  • Höllrigl V., Hollmann F., Kleeb A., Otto K. and Schmid A. (2005)
    Enantioselective cell-free bioreductions coupled ton non-enzymatic cofactor regeneration.
    Biotrans 2005 - 7th International Symposium on Biocatalysis and Biotransformations, 3. - 8. July 2005, Delft, Netherlands


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